全天PK10北京计划在线免费计划

网站地图 联系我们 │ │ 内部网English |
全天PK10北京计划在线免费计划 所况简介 机构设置 科研队伍 院地合作 党群园地 国际交流 博士后 研究生教育 信息公开
科研进展
成果报道
最新重要论文(影响因子PNAS及以上)
发表论文数据库
所级学术报告
科学成果
发表论文
专著
专利
获奖
专题
所史丛书
所庆专辑
建所50周年画册
现在位置:全天PK10北京计划在线免费计划 > 科研进展 > 最新重要论文(影响因子PNAS及以上)
Programmable base editing of mutated TERT promoter inhibits brain tumour growth, Nat Cell Biol, 17 Feb 2020
2020-02-17 | 【     】【打印】【关闭

Nature Cell Biology全天PK10北京计划在线免费计划17 February, 2020,DOI:

Programmable base editing of mutated TERT promoter inhibits brain tumour growth

全天PK10北京计划在线免费计划Xinjian Li, Xu Qian, Bin Wang, Yan Xia, Yanhua Zheng, Linyong Du, Daqian Xu, Dongming Xing, Ronald A. DePinho & Zhimin Lu

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR interference and programmable base editing have transformed the manipulation of eukaryotic genomes for potential therapeutic applications1,2,3,4. Here, we exploited CRISPR interference and programmable base editing to determine their potential in editing a TERT gene promoter-activating mutation, which occurs in many diverse cancer types, particularly glioblastoma5,6,7,8全天PK10北京计划在线免费计划. Correction of the ?124C>T TERT promoter mutation to ?124C was achieved using a single guide RNA (sgRNA)-guided and catalytically impaired Campylobacter jejuni CRISPR-associated protein 9-fused adenine base editor (CjABE). This modification blocked the binding of members of the E26 transcription factor family to the TERT promoter, reduced TERT transcription and TERT protein expression, and induced cancer-cell senescence and proliferative arrest. Local injection of adeno-associated viruses expressing sgRNA-guided CjABE inhibited the growth of gliomas harbouring TERT-promoter mutations. These preclinical proof-of-concept studies establish the feasibility of gene editing as a therapeutic approach for cancer and validate activated TERT-promoter mutations as a cancer-specific therapeutic target.

文章链接:

相关报道:http://www.hnshb.com/kyjz/zxdt/202002/t20200218_5501791.html

 

       
版权所有:中国科学院生物物理研究所     京ICP备05002792号 京公网安备 110402500011 号
地址:北京市朝阳区大屯路15号(100101) 电话:010-64889872
意见反馈联系人:侯文茹 电子邮件:houwenru@ibp.ac.cn